Anti-p70 S6 Kinase (Ser398) Antibody

Our Anti-p70 S6 Kinase (Ser398) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila p70 S6 Kinase (Ser398) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

P70 S6 kinase (p70 S6K) is activated in a signaling pathway that includes mTOR and is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (Xio et al., 2009) . p70 S6K is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains and subsequently phosphorylates specifically ribosomal protein S6 (Saitoh et al., 2002) . Phosphorylation of Thr-229 in the catalytic domain and Thr-389 in the linker domain are most critical for kinase function. Inhibition of p70 activity inhibits the entry into S phase of the cell cycle and exhibits cell cycle arrest at G0/G1 phase, suggesting that the activation of p70 S6k plays an obligatory role in mediating mitogenic signals during cell activation (Xio et al., 2009) .

Synonyms

70 kDa ribosomal protein S6 kinase 2 antibody, EC 2.7.11.1 antibody, KS6B2_HUMAN antibody, p70 beta antibody, p70 ribosomal S6 kinase beta antibody, p70 S6 kinase beta antibody, p70 S6K-beta antibody, p70 S6KB antibody, p70 S6Kbeta antibody, p70 (S6K) beta antibody, p70-beta antibody, p70-S6K 2 antibody, P70S6K2 antibody, p70S6Kb antibody, Ribosomal protein S6 kinase 70kDa polypeptide 2 antibody, Ribosomal protein S6 kinase B2 antibody, Ribosomal protein S6 kinase beta 2 antibody, Ribosomal protein S6 kinase beta-2 antibody, Rps6kb2 antibody, S6 kinase related kinase antibody, S6 kinase-related kinase antibody, S6K beta 2 antibody, S6K beta antibody, S6K-beta antibody, S6K-beta-2 antibody, S6K2 antibody, Serine/threonine protein kinase 14 beta antibody, Serine/threonine-protein kinase 14B antibody, SRK antibody, STK14B antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

S6K

UniProt

H1ZYE3

Modification Site

Ser398

Host

Rabbit

Reactivity

Drosophila

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr398 of Drosophila p70 S6K protein, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Drosophila

Target

P70 S6 Kinase (Ser398)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~70 kDa p70 S6K protein phosphorylated at Thr398. Immunolabeling is blocked by preadsorption with the Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.