Anti-Ribosomal protein S6 (Ser244) Antibody

Our Anti-Ribosomal protein S6 (Ser244) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse Ribosomal protein S6 (Ser244) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5'-m7 GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011) . In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011) . Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-γ), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K) –Akt–mTOR pathway (Chen and Dittmer 2011) .

Synonyms

40S ribosomal protein S6 antibody, Air8 antibody, NP33 antibody, Phosphoprotein NP33 antibody, Pp30 antibody, Ribosomal protein S6 antibody, RP S6 antibody, rps6 antibody, RPS6 antibody, RS6 antibody, RS6_HUMAN antibody, S6 antibody, S6 Ribosomal Protein antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

RPS6

NCBI Gene ID

20104

UniProt

P62754

Modification Site

Ser244

Host

Rabbit

Reactivity

Human and Mouse

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 of mouse rpS6, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Mouse

Target

Ribosomal protein S6 (Ser244)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:500

Specificity

Specific for endogenous levels of the ~28 kDa rpS6 protein phosphorylated at Ser244. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.