Anti-REDD1 (Thr23/25) Antibody

Our Anti-REDD1 (Thr23/25) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human REDD1 (Thr23/25) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli (Schwarzer et al, 2005) . REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009) . It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr-25, Thr-23 and Ser-19 through the activity of GSK3b (Katiyar et al, 2009) .

Synonyms

DDIT4 antibody, DDIT4_HUMAN antibody, Dig2 antibody, DNA damage inducible transcript 4 antibody, DNA damage inducible transcript 4 protein antibody, DNA damage-inducible transcript 4 protein antibody, FLJ20500 antibody, HIF 1 responsive protein RTP801 antibody, HIF 1 responsive RTP801 antibody, HIF-1 responsive protein RTP801 antibody, Protein regulated in development and DNA damage response 1 antibody, REDD-1 antibody, REDD1 antibody, RTP801 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

DDIT4

NCBI Gene ID

54541

UniProt

Q9NX09

Modification Site

Thr23,25

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr23/25 of human REDD1, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

REDD1 (Thr23/25)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:500

Specificity

Specific for endogenous levels of the ~34 kDa REDD1 phosphorylated at Thr23/25. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.