Anti-PTPH1 (Ser459) Antibody

Our Anti-PTPH1 (Ser459) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse PTPH1 (Ser459) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010) . Ras has been demonstrated to increase both p38γ and PTPH1 protein expression, and there is a coupling of increased p38γ and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010) . Phosphorylation of PTPH1 at Ser-459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012) . Additionally, phosphorylation of PTPH1 Ser-459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012) .

Synonyms

Cytoskeletal-associated protein tyrosine phosphatase antibody, protein tyrosine phosphatase H1 antibody, protein tyrosine phosphatase non-receptor type 3 antibody, Protein-tyrosine phosphatase H1 antibody, PTN3_HUMAN antibody, PTP-H1 antibody, PTPH1 antibody, PTPN3 antibody, Tyrosine-protein phosphatase non-receptor type 3 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

PTPH1

NCBI Gene ID

5774

UniProt

P26045

Modification Site

Ser459

Host

Rabbit

Reactivity

Human and Mouse

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser459 of human PTPH1, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

PTPH1 (Ser459)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

104

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~104 kDa PTPH1 protein phosphorylated at Ser459. The immunolabeling is completely eliminated by treatment with λ-phosphatase.