Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody

Our Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Potassium Chloride Cotransporter (KCC2) (Ser940) and is antigen affinity purified from pooled serum. It is great for use in WB, IP.

Product Specifications

Background

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser-940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane (Lee et al., 2007) .

Synonyms

Electroneutral potassium chloride cotransporter 2 antibody, Electroneutral potassium-chloride cotransporter 2 antibody, Erythroid K Cl cotransporter 2 antibody, Furosemide sensitive K Cl cotransporter antibody, hKCC2 antibody, K-Cl cotransporter 2 antibody, KCC 2 antibody, KCC2 antibody, KIAA1176 antibody, Neuronal K Cl cotransporter antibody, Neuronal K-Cl cotransporter antibody, Potassium Chloride Cotransporter antibody, Potassium chloride transporter 5 antibody, rKCC2 antibody, S12A5 antibody, S12A5_HUMAN antibody, SLC12A5 antibody, Solute carrier family 12 (potassium chloride transporter) member 5 antibody, Solute carrier family 12 member 5 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

KCC2

NCBI Gene ID

171373

UniProt

Q63633

Modification Site

Ser940

Host

Rabbit

Reactivity

Mouse and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Rat

Target

Potassium Chloride Cotransporter (KCC2) (Ser940)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, IP

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

135

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

IP Dilution

40ul of antibody per 1mg lysate

Specificity

Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Ser940. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.