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Anti-Polo-Like Kinase Kinase (Ser482,486,490) Antibody

Our Anti-Polo-Like Kinase Kinase (Ser482,486,490) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat and Xenopus Polo-Like Kinase Kinase (Ser482,486,490) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser-482, Ser-486 and Ser-490) . Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser-482,486,490 in PLKK.

Synonyms

Polo like kinase kinase 1 antibody, Serine/threonine kinase 10 antibody, stk10 a antibody, xPlkk1 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

PLKK1

NCBI Gene ID

380481

UniProt

B7ZR30

Modification Site

Ser482,486,490

Host

Rabbit

Reactivity

Rat and Xenopus

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 of Xenopus polo-like kinase kinase (PLKK), conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Xenopus

Target

Polo-Like Kinase Kinase (Ser482,486,490)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

120

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~120k PLKK protein phosphorylated at Ser482,486,490.

Formulation

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Quality Control

Western blots performed on each lot.

Species

Human, Mouse, Zebrafish

Isotype

IgG

Frequently Asked Questions

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