Anti-p62 (Ser28) Antibody

Our Anti-p62 (Ser28) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human p62 (Ser28) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

P62, also known as sequestosome1 (SQSTM1), is a shuttle protein transporting polyubiquitinated proteins for both proteasomal and lysosomal degradation. p62 is an integral component of inclusions in brains of various neurodegenerative disorders, including Alzheimer disease (AD) neurofibrillary tangles (NFTs) and Lewy bodies in Parkinson disease (Nogalaska et al., 2009) . p62 plays an important role in the protection of cells from the toxicity of misfolded proteins by enhancing aggregate formation especially in the later stages (Nakaso et al., 2004) . Phosphorylation of Ser-28 has recently been demonstrated to be related to the pathogenesis of Parkinson’s disease.

Synonyms

A170 antibody, DMRV antibody, EBI 3 associated protein of 60 kDa antibody, EBI 3 associated protein p60 antibody, EBI3 associated protein of 60 kDa antibody, EBI3 associated protein p60 antibody, EBI3-associated protein of 60 kDa antibody, EBIAP antibody, FTDALS3 antibody, MGC127197 antibody, ORCA antibody, OSF-6 antibody, Osi antibody, OSIL antibody, Oxidative stress induced like antibody, p60 antibody, p62 antibody, p62B antibody, Paget disease of bone 3 antibody, PDB 3 antibody, PDB3 antibody, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody, Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody, PKC-zeta-interacting protein antibody, Protein kinase C-zeta-interacting protein antibody, Sequestosome 1 antibody, Sequestosome-1 antibody, SQSTM 1 antibody, SQSTM_HUMAN antibody, Sqstm1 antibody, STAP antibody, STONE14 antibody, Ubiquitin binding protein p62 antibody, Ubiquitin-binding protein p62 antibody, ZIP 3 antibody, ZIP antibody, ZIP3 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

SQSTM1

NCBI Gene ID

8878

UniProt

Q13501

Modification Site

Ser28

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser28 of human p62, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

P62 (Ser28)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~48 kDa p62 protein phosphorylated at Ser28. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.