Anti-Nuclei Antibody

Our Anti-Nuclei primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Nuclei and is concentrated tissue culture supernatant. It is great for use in IHC, ICC.

Product Specifications

Background

This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.

Synonyms

HNA antibody, Human Nuclear Antigen antibody, HuNu antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

Marker for human cells in xenographic research

Host

Mouse

Reactivity

Human

Immunogen

Human cell homogenate.

Immunogen Type

Human

Target

Nuclei

Clonality

Monoclonal

Clone

235-1

Conjugation

Unconjugated

Applications

ELISA, IHC, ICC

Concentration

1 mg/ml

Format

Concentrated tissue culture supernatant

Form

Liquid

Buffer

Concentrated tissue culture supernatent

Shipping Conditions

Blue Ice

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

IHC Dilution

1:100

Specificity

Specific for endogenous levels of human nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.