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Anti-Neurofilament NF-M Antibody

Our Anti-Neurofilament NF-M primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, chicken, human, mouse, and rat Neurofilament NF-M and is total IgY fraction. It is great for use in WB, IHC, ICC.

Product Specifications

Background

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (Harris et al., 1991) . NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (Hu et al., 2002) .

Synonyms

150 kDa Neurofilament Medium antibody, 160 kDa neurofilament protein antibody, Medium polypeptide 150kDa antibody, NEF3 antibody, Neurofilament 3 antibody, Neurofilament 3 medium antibody, Neurofilament medium polypeptide antibody, Neurofilament protein M antibody, Neurofilament triplet M protein antibody, Neurofilament-3 (150 kD medium) antibody, NF-M antibody, NFM antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

NEFM

NCBI Gene ID

24588

UniProt

P12839

Host

Chicken

Reactivity

Bovine, Chicken, Human, Mouse, and Rat

Immunogen

Preparation containing the extreme C-terminus of rat NF-M, expressed in and purified from E. Coli.

Immunogen Type

Rat

Target

Neurofilament NF-M

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

21.5 mg/ml

Format

Total IgY fraction

Form

Liquid

Buffer

PBS + 10 mM NaN3.

Molecular Weight

145

Shipping Conditions

Blue Ice

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

IHC Dilution

1:1000

WB Dilution

1:5000

Specificity

Specific for endogenous levels of the ~145 kDa Neurofilament M protein.

Formulation

Total IgY fraction

ICC Dilution

1:1000

Quality Control

Western blots performed on each lot.

Isotype

IgY

Frequently Asked Questions

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