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Anti-Neurofilament NF-H Antibody

Our Anti-Neurofilament NF-H primary antibody from PhosphoSolutions is chicken polyclonal. It detects all known mammal and chicken Neurofilament NF-H and is total IgY fraction. It is great for use in WB, IHC, ICC.

Product Specifications

Background

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (Harris et al., 1991) . NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (Mendonca et al., 2005) and Alzheimer's disease (Hu et al., 2002) .

Synonyms

200 kDa neurofilament protein antibody, CMT2CC antibody, Nefh antibody, Neurofilament heavy polypeptide 200kDa antibody, Neurofilament heavy polypeptide antibody, Neurofilament triplet H protein antibody, NF H antibody, NF-H antibody, NFH antibody, NFH_HUMAN antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

NEFH

NCBI Gene ID

4744

UniProt

P12036

Host

Chicken

Reactivity

All Known Mammal and Chicken

Immunogen

Purified bovine NF-H

Immunogen Type

Bovine

Target

Neurofilament NF-H

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

30 mg/ml

Format

Total IgY fraction

Form

Liquid

Buffer

PBS + 10 mM NaN3.

Molecular Weight

200

Shipping Conditions

Blue Ice

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

IHC Dilution

1:5000-1:25,000

WB Dilution

1:50000

Specificity

Specific for endogenous levels of the ~200 kDa Neurofilament H protein.

Formulation

Total IgY fraction

ICC Dilution

1:25,000

Quality Control

Western blots performed on each lot.

Isotype

IgY

Frequently Asked Questions

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