Anti-NCC (Thiazide sensitive NaCl cotransporter) (Thr53) Antibody

Our Anti-NCC (Thiazide sensitive NaCl cotransporter) (Thr53) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NCC (Thiazide sensitive NaCl cotransporter) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Specifications

Background

The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015) . Phosphorylation of NCC at Thr-53, Thr-58, and Ser-71 is an essential mediator of NCC function (Rosenbaek et al., 2014) . NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015) .

Synonyms

FLJ96318 antibody, Na Cl cotransporter antibody, Na Cl symporter antibody, Na-Cl symporter antibody, NaCl electroneutral thiazide sensitive cotransporter antibody, NCCT antibody, SLC12A3 antibody, S12A3_HUMAN antibody, slc12a3 antibody, Solute carrier family 12 (sodium/chloride transporters) member 3 antibody, Solute carrier family 12 member 3 antibody, Thiazide sensitive Na Cl cotransporter antibody, Thiazide sensitive sodium chloride cotransporter antibody, Thiazide-sensitive sodium-chloride cotransporter antibody, TSC antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

SLC12A3

NCBI Gene ID

20497

UniProt

P59158

Modification Site

Thr53

Host

Rabbit

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of mouse NCC, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Mouse

Target

NCC (Thiazide sensitive NaCl cotransporter)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

160

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

IHC Dilution

1:100-1:10,000

WB Dilution

1:1000-1:6000

Specificity

Specific for endogenous levels of the ~160 kDa NCC protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-phosphatase.