Anti-Munc-18 (Ser 513) Antibody

Our Anti-Munc-18 (Ser 513) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Munc-18 (Ser 513) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Product Specifications

Background

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005) . Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004) . The function of Unc-18 is thought to be regulated by PKC phosphorylation of Ser-515 in C. Elegans (Sassa et al., 1996) . Ser-513 is the mammalian homologue of Ser-515 in C. Elegans.

Synonyms

FLJ37475 antibody, Munc 18 1 antibody, Munc 18a antibody, MUNC18 1 antibody, N-Sec1 antibody, Neuronal SEC1 antibody, NSec1 antibody, p67 antibody, Protein unc-18 homolog 1 antibody, Protein unc-18 homolog A antibody, Rb sec1 antibody, RBSEC1 antibody, STXB1_HUMAN antibody, STXBP1 antibody, Syntaxin binding protein 1 antibody, Syntaxin-binding protein 1 antibody, Unc 18 homolog antibody, Unc 18A antibody, Unc-18A antibody, Unc18 1 antibody, UNC18 antibody, Unc18-1 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

STXBP2

NCBI Gene ID

81804

UniProt

Q62753

Modification Site

Ser513

Host

Rabbit

Reactivity

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser513 of rat Munc-18, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Rat

Target

Munc-18 (Ser 513)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~65 kDa Munc-18 protein phosphorylated at Ser513. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.