Anti-Mnk2b Antibody

Our Anti-Mnk2b rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Mnk2b and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

MAP kinase-interacting kinases Mnk1 and Mnk2, are activated by the MAP kinases, ERK and p38 (Waskiewicz et al., 1997) . Additionally, Mnk1 and Mnk2 have been shown to phosphorylate the translation initiation factor eIF4E (Fukunaga and Hunter 1997; Waskiewicz et al., 1997) . Alternative splicing of the MKNK2 gene results in two isoforms with different C-termini: one that has a MAPK-binding domain, Mnk2a, and one that does not, Mnk2b (Parra et al., 2005) . Recent studies have indicated that Mnk2a has tumor suppressive activity while Mnk2b is pro-oncogenic (Maimon et al., 2014) .

Synonyms

MADS box transcription enhancer factor 2 antibody, MDS1 EVI1 antibody, Mef2b antibody, MEF2B_HUMAN antibody, Myocyte enhancer factor 2B antibody, Myocyte specific enhancer factor 2B antibody, Myocyte-specific enhancer factor 2B antibody, PRDM3 antibody, RSRFR2 antibody, Serum response factor like protein 2 antibody, Serum response factor-like protein 2 antibody, XMEF2 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

MKNK2

NCBI Gene ID

2872

UniProt

Q9HBH9

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide from the C-terminal region, specific to the human Mnk2b isoform. The sequence of the antigen has no homology with Mnk1 or Mnk2a.

Immunogen Type

Human

Target

Mnk2b

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~47 kDa Mnk2b protein. Immunolabeling is blocked by preadsorption with the Mnk2b peptide used as antigen, but not by Mnk2a peptide.