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Anti-Mitochondria Antibody

Our Anti-Mitochondria primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Mitochondria and is Protein A purified. It is great for use in WB, IHC, ICC.

Product Specifications

Background

Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006) . In addition, mitochondria have been shown to play a role in apoptosis (Green 1998) . This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Synonyms

Mitochondrial marker

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Host

Mouse

Reactivity

Human

Immunogen

Human cell homogenate

Immunogen Type

Human

Target

Mitochondria

Clonality

Monoclonal

Clone

113-1

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

1 mg/ml

Format

Protein A Purified

Form

Liquid

Buffer

PBS

Molecular Weight

60

Shipping Conditions

Blue Ice

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

IHC Dilution

1:100-1:800

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~60 kDa human mitochondria. The antibody works on acetone fixed and 2% paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2% paraformaldehyde fixed (5-10 minutes) cryostat sections.

Formulation

Protein A purified culture supernatant

ICC Dilution

1:100-1:800

Quality Control

Western blots performed on each lot.

Isotype

IgG1

Curated Selection

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