Anti-MEK5 (Ser311, Thr315) Antibody

Our Anti-MEK5 (Ser311, Thr315) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat MEK5 (Ser311, Thr315) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999) . An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003) . Dual phosphorylation of Ser-311 and Thr-315 have been implicated in cell proliferation (Cameron et al., 2004) .

Synonyms

Dual specificity mitogen activated protein kinase kinase 5 antibody, Dual specificity mitogen-activated protein kinase kinase 5 antibody, EC 2.7.12.2 antibody, HsT17454 antibody, MAP kinase kinase 5 antibody, MAP kinase kinase MEK5b antibody, MAP2K5 antibody, MAPK/ERK kinase 5 antibody, MAPKK 5 antibody, MAPKK5 antibody, MEK 5 antibody, mitogen-activated protein kinase kinase 5 antibody, MKK5 antibody, MP2K5_HUMAN antibody, PRKMK5 antibody, Protein kinase mitogen-activated kinase 5 antibody, SAPKK5 antibody, SKK5 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

MAP2K5

NCBI Gene ID

31068

UniProt

Q62862

Modification Site

Ser311, Thr315

Host

Rabbit

Reactivity

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 of rat MEK5, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Rat

Target

MEK5 (Ser311, Thr315)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~49 kDa MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.