Anti-MeCP2 (Ser421) Antibody

Our Anti-MeCP2 (Ser421) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat MeCP2 (Ser421) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998) . Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999) . RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser-421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment (Hutchinson et al., 2012) .

Synonyms

AUTSX 3 antibody, AUTSX3 antibody, DKFZp686A24160 antibody, Mbd 5 antibody, Mbd5 antibody, MECP 2 antibody, MeCP 2 protein antibody, MeCP-2 protein antibody, Mecp2 antibody, MECP2_HUMAN antibody, Methyl CpG binding protein 2 (Rett syndrome) antibody, Methyl CpG binding protein 2 antibody, Methyl-CpG-binding protein 2 antibody, MRX 16 antibody, MRX 79 antibody, MRX16 antibody, MRX79 antibody, MRXS 13 antibody, MRXS13 antibody, MRXSL antibody, PPMX antibody, RS antibody, RTS antibody, RTT antibody, WBP 10 antibody, WBP10 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

MECP2

NCBI Gene ID

17257

UniProt

Q9Z2D6

Modification Site

Ser421

Host

Rabbit

Reactivity

Mouse and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 of mouse MeCP2, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Mouse

Target

MeCP2 (Ser421)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.