Anti-MARCKS Antibody
Our Anti-MARCKS primary antibody from PhosphoSolutions is chicken polyclonal. It detects human MARCKS and is total IgY fraction. It is great for use in WB, IHC, ICC.
Product Specifications
Background
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentratedin synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992) . Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al.1995) . MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser-152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994) .
Synonyms
80 kDa protein antibody, 80K L antibody, 80K L protein antibody, 80K-L protein antibody, 80KL antibody,81 kDa protein, light chain antibody, light chain antibody, MACS antibody, MARCKS antibody, MARCS antibody, MARCS_HUMAN antibody, MGC52672 antibody, myristoylated alanine rich C kinase substrate antibody, Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody, Myristoylated alanine rich protein kinase C substrate antibody, Myristoylated alanine-rich C-kinase substrate antibody, Phosphomyristin antibody, PKCSL antibody, PRKCSL antibody, protein kinase C substrate 80 kDa protein light chain antibody, Protein kinase C substrate antibody
Other Statements
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Gene Name
MARCKS
NCBI Gene ID
4082
UniProt
P29966
Host
Chicken
Reactivity
Human
Immunogen
Recombinant human MARCKS expressed in and purified from E. coli
Immunogen Type
Human
Target
MARCKS
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
ELISA, WB, IHC, ICC
Concentration
Lot Dependent
Format
Total IgY fraction
Form
Liquid
Buffer
PBS + 10 mM NaN3.
Molecular Weight
80
Shipping Conditions
Blue Ice
Storage Conditions
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
IHC Dilution
1:500-1:1000
WB Dilution
1:10000
Specificity
Specific for endogenous levels of the ~80 kDa MARCKS protein.
Formulation
Total IgY fraction
ICC Dilution
1:500-1:5000
Quality Control
Western blots performed on each lot.
Species
Non-Human Primate
Isotype
IgY
Curated Selection
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