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Anti-MARCKS Antibody

Our Anti-MARCKS primary antibody from PhosphoSolutions is chicken polyclonal. It detects human MARCKS and is total IgY fraction. It is great for use in WB, IHC, ICC.

Product Specifications

Background

Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentratedin synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992) . Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al.1995) . MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser-152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994) .

Synonyms

80 kDa protein antibody, 80K L antibody, 80K L protein antibody, 80K-L protein antibody, 80KL antibody,81 kDa protein, light chain antibody, light chain antibody, MACS antibody, MARCKS antibody, MARCS antibody, MARCS_HUMAN antibody, MGC52672 antibody, myristoylated alanine rich C kinase substrate antibody, Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody, Myristoylated alanine rich protein kinase C substrate antibody, Myristoylated alanine-rich C-kinase substrate antibody, Phosphomyristin antibody, PKCSL antibody, PRKCSL antibody, protein kinase C substrate 80 kDa protein light chain antibody, Protein kinase C substrate antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

MARCKS

NCBI Gene ID

4082

UniProt

P29966

Host

Chicken

Reactivity

Human

Immunogen

Recombinant human MARCKS expressed in and purified from E. coli

Immunogen Type

Human

Target

MARCKS

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

Lot Dependent

Format

Total IgY fraction

Form

Liquid

Buffer

PBS + 10 mM NaN3.

Molecular Weight

80

Shipping Conditions

Blue Ice

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

IHC Dilution

1:500-1:1000

WB Dilution

1:10000

Specificity

Specific for endogenous levels of the ~80 kDa MARCKS protein.

Formulation

Total IgY fraction

ICC Dilution

1:500-1:5000

Quality Control

Western blots performed on each lot.

Species

Non-Human Primate

Isotype

IgY

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