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Anti-Ki-67 Antibody

Our Anti-Ki-67 Antibody primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Ki-67 and is Protein G purified. It is great for use in WB, IHC, ICC.

Product Specifications

Background

Ki-67 is a nuclear protein that is considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000) . Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018) .

Synonyms

Antigen identified by monoclonal antibody Ki 67 antibody, Antigen identified by monoclonal antibody Ki-67 antibody, Antigen KI-67 antibody, Antigen KI67 antibody, Antigen Ki67 antibody, Ki67 antibody, KI67_HUMAN antibody, KIA antibody, Marker of proliferation Ki-67 antibody, MIB 1 antibody, MIB antibody, MKI67 antibody, PPP1R105 antibody, Proliferation marker protein Ki-67 antibody, Proliferation related Ki 67 antigen antibody, Protein phosphatase 1 regulatory subunit 105 antibody, RP11-380J17.2 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

MKI67

NCBI Gene ID

4288

UniProt

P46013

Host

Mouse

Reactivity

Human

Immunogen

Recombinant construct containing the N-terminal 300 amino acids of the human sequence expressed in and purified from E. coli.

Immunogen Type

Human

Target

Ki-67

Clonality

Monoclonal

Clone

6G3

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

1 mg/mL

Format

Protein G Purified

Form

Liquid

Buffer

PBS + 50% glycerol and 5 mm NaN3

Molecular Weight

345-395

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:2000

Specificity

Specific for endogenous levels of the ~345 & 395 kDa Ki-67 isoforms. Additional bands are thought to be breakdown products of these 2 isoforms.

Formulation

Protein G purified culture supernatant

ICC Dilution

1:2000-1:5000

Quality Control

Western blots performed on each lot.

Isotype

IgG1

Curated Selection

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