Anti-Interferon-α Receptor, Type I, Subunit I (Ser535,539) Antibody

Our Anti-Interferon-α Receptor, Type I, Subunit I (Ser535,539) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Interferon-α Receptor, Type I, Subunit I (Ser535,539) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002) . These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1) . Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine-535 and Ser-539 in the IFNAR1 (Kumar et al., 2003) .

Synonyms

Alpha-Type Antiviral Protein, AVP, Beta-Type Antiviral Protein, CRF2-1, Cytokine Receptor Class-II Member 1, Cytokine Receptor Family 2 Member 1, IFN-Alpha/Beta Receptor 1, IFNBR, IFN-Alpha-REC, IFNAR, IFN-R-1, IFRC, Interferon Alpha/Beta Receptor 1, Interferon-Alpha/Beta Receptor Alpha Chain, Interferon Alpha And Beta Receptor Subunit 1, Interferon (Alpha, Beta And Omega) Receptor 1, Interferon-Beta Receptor 1, Interferon Receptor 1, Type I Interferon Receptor 1

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

IFNAR1

NCBI Gene ID

3654

UniProt

P17181

Modification Site

Ser535,539

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 of human interferon-α, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

Interferon-α Receptor, Type I, Subunit I (Ser535,539)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

110-130

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for IFNAR1 protein phosphorylated at Ser535,539. Note: the molecular weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110 kDa - 130 kDa.