Anti-HDAC2 (Ser394) Antibody

Our Anti-HDAC2 (Ser394) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat HDAC2 (Ser394) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

Histone Deacetylase 2 (HDAC2) is part of a family of histone deacetylases that are responsible for deacetylation of lysine residues in the histone core. HDAC2 is classified as a class I histone deacetylase and is ubiquitously expressed throughout the body (Kee et al, 2008) . It has been shown that HDAC2 plays an important role in cardiac hypertrophy (Eom et al, 2011) . Phosphorylation of Ser-394 is responsible for the hypertrophy-associated activation of HDAC2, whereas intrinsic basal activity is maintained by phosphorylation of Ser-422 and Ser-424 (EOM et al, 2011) .

Synonyms

D10Wsu179e antibody, HD 2 antibody, HD2 antibody, HDAC 2 antibody, Hdac2 antibody, HDAC2_HUMAN antibody, Histone deacetylase 2 (HD2) antibody, Histone deacetylase 2 antibody, OTTHUMP00000017046 antibody, OTTHUMP00000227077 antibody, OTTHUMP00000227078 antibody, RPD3 antibody, transcriptional regulator homolog RPD3 antibody, YAF1 antibody, YY1 associated factor 1 antibody, YY1 transcription factor binding protein antibody, Yy1bp antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

HDAC2

NCBI Gene ID

3066

UniProt

Q92769

Modification Site

Ser394

Host

Rabbit

Reactivity

Mouse and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser394 of human HDAC2, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

HDAC2 (Ser394)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~55 kDa HDAC2 protein phosphorylated at Ser394. Immunolabeling is completely eliminated by treatment with λ-phosphatase.