Anti-Glycogen synthase kinase 3 B (GSK3B) (Ser9) Antibody

Our Anti-Glycogen synthase kinase 3 B (GSK3B) (Ser9) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Glycogen synthase kinase 3 B (GSK3B) (Ser9) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3α and GSK3β. Specifically, GSK3β has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998) . It has been suggested that Ser-9 phosphorylation underlies the inhibition of GSK3β by insulin (Sutherland et al., 1993) .

Synonyms

Glycogen Synthase Kinase 3 Beta antibody, Glycogen synthase kinase-3 beta antibody, GSK 3 beta antibody, GSK-3 beta antibody, GSK3B antibody, GSK3B_HUMAN antibody, GSK3beta isoform antibody, Serine/threonine-protein kinase GSK3B antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

GSK3B

NCBI Gene ID

84027

UniProt

P18266

Modification Site

Ser9

Host

Rabbit

Reactivity

Mouse and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat GSK3β, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Rat

Target

Glycogen synthase kinase 3 B (GSK3B) (Ser9)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for the ~46 kDa GSK3β protein phosphorylated at Ser9. Also weakly labels the ~51 kDa GSK3β band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.