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Anti-EWS Antibody

Our Anti-EWS primary antibody from PhosphoSolutions is mouse monoclonal. It detects canine, human, mouse, and rat EWS and is Protein G purified. It is great for use in WB, IHC, ICC.

Product Specifications

Background

The Ewing Sarcoma Breakpoint Region 1 (EWSR1) gene encodes the EWS protein, which along with FUS and TAF15 comprises the FET family of RNA-binding proteins. EWS is primarily expressed in the nucleus, but has also been found in the cytoplasm of secretory cell types (Andersson et al., 2008) . Defects of EWSR1 are known to cause Ewing sarcoma and have been implicated in amyotrophic lateral sclerosis (ALS) (Svetoni et al., 2016) . Recently, EWS has been shown to directly interact with oncogenic ETS transcription factors in the promotion of prostate cancer (Kedage et al., 2016) .

Synonyms

4 Ewing sarcoma breakpoint region 1 protein antibody, Ewing sarcoma breakpoint region 1 antibody, Ewing sarcoma breakpoint region 1 protein antibody, Ewings sarcoma EWS Fli1 type 1 oncogene antibody, EWS antibody, EWS oncogene antibody, EWS RNA binding protein 1 antibody, EWS_HUMAN antibody, EWSR 1 antibody, Ewsr1 antibody, EWSR1 protein antibody, RNA binding protein EWS antibody, RNA-binding protein EWS antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

EWSR1

NCBI Gene ID

2130

UniProt

Q01844

Host

Mouse

Reactivity

Canine, Human, Mouse, and Rat

Immunogen

Full length recombinant human EWS expressed in and purified from E. coli.

Immunogen Type

Human

Target

EWS

Clonality

Monoclonal

Clone

5H7

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

Lot Dependent

Format

Protein G Purified

Form

Liquid

Buffer

PBS + 50% glycerol and 5 mm NaN3

Molecular Weight

85

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Datasheet

https://cdn.shopify.com/s/files/1/0520/9063/4413/files/540-EWS.pdf

IHC Dilution

1:1000

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~85 kDa EWS protein.

Formulation

Protein G purified from culture supernatant.

ICC Dilution

1:1000

Quality Control

Western blots performed on each lot.

Isotype

IgG2b

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