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Anti-ERK1/2 (MAPK) (Thr202/Tyr204) Antibody

Our Anti-ERK1/2 (MAPK) (Thr202/Tyr204) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat ERK1/2 (MAPK) (Thr202/Tyr204) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Product Specifications

Background

Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002) . The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993) .

Synonyms

ERK 1 antibody, ERK 2 antibody, ERK-2 antibody, ERK1 antibody, erk1/2 antibody, ERK2 antibody, ERT1 antibody, ERT2 antibody, Extracellular signal regulated kinase 1 antibody, Extracellular signal-regulated kinase 2 antibody, MAP kinase 1 antibody, MAP kinase 2 antibody, MAP kinase isoform p42 antibody, MAP kinase isoform p44 antibody, MAPK 1 antibody, MAPK 2 antibody, MAPK 3 antibody, Mapk1 antibody, MAPK2 antibody, MAPK3 antibody, Mitogen-activated protein kinase 1 antibody, Mitogen-activated protein kinase 2 antibody, MK01_HUMAN antibody, p38 antibody, p40 antibody, p41 antibody, p42-MAPK antibody, PRKM 2 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

MAPK1/2

NCBI Gene ID

116590

UniProt

P63086

Modification Site

Thr202, Tyr204

Host

Rabbit

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 of rat ERK/MAPK conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Rat

Target

ERK/MAPK (Thr202/Tyr204)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

42/44

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

IHC Dilution

1:100-1:500

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~42 - 44 kDa ERK/MAPK protein phosphorylated at Thr202 and Tyr204. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Formulation

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

ICC Dilution

1:100-1:250

Quality Control

Western blots performed on each lot.

Species

Bovine, Canine, Chicken, Non-Human Primate, Xenopus, Zebrafish

Isotype

IgG

Frequently Asked Questions

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