Anti-ERK/MAPK Antibody
Our Anti-ERK/MAPK rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat ERK/MAPK and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.
Product Specifications
Background
Synonyms
Other Statements
Gene Name
MAPK1/2
NCBI Gene ID
116590
UniProt
P63086
Host
Rabbit
Reactivity
Human, Mouse, and Rat
Immunogen
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat ERK, conjugated to keyhole limpet hemocyanin (KLH) .
Immunogen Type
Rat
Target
ERK/MAPK
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
ELISA, WB, ICC
Concentration
Lot Dependent
Format
Antigen Affinity Purified from Pooled Serum
Form
Liquid
Buffer
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Molecular Weight
42/44
Shipping Conditions
Blue Ice
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
WB Dilution
1:1000
Specificity
Specific for endogenous levels of the ~42-44 kDa ERK/MAPK protein. Immunolabeling is blocked by preadsorption with the peptide used as antigen.
Formulation
Prepared from pooled rabbit serum by affinity purification.
ICC Dilution
1:250
Quality Control
Western blots performed on each lot.
Isotype
IgG
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