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Anti-eIF4A2 Antibody

Our Anti-eIF4A2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse eIF4A2 and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

The eIF4A, eukaryotic initiation factor 4A, are a set of proteins, eIF4A1, eIF4A2, and eIF4A3, that belong to the extensive DEAD-box RNA helicase family (Weinstein et al., 1997) . They are involved in many aspects of RNA metabolism by virtue of their RNA-binding capacity and ATPase activity (Lu et al., 2014) . eIF4A2 is a highly conserved gene for one of the protein-synthesis initiation factors involved in the binding of mRNA to the ribosome (Gingras et al., 1999) . In humans eIF4A2 is expressed in all normal tissues, though highly expressed in skeletal muscle (Sudo et al., 1995) . Studies suggest eIF4A2 may play a role in some cancers, specifically breast and lung cancer, along with melanoma (Shaoyan et al., 2013) .

Synonyms

ATP dependent RNA helicase eIF4A 2 antibody, ATP-dependent RNA helicase eIF4A-2 antibody, BM-010 antibody, DDX2B antibody, eIF 4A II antibody, eIF-4A-II antibody, EIF4A antibody, eIF4A II antibody, eIF4A-II antibody, EIF4A2 antibody, EIF4F antibody, Eukaryotic initiation factor 4A II antibody, Eukaryotic initiation factor 4A-II antibody, eukaryotic translation initiation factor 4A isoform 2 antibody, Eukaryotic translation initiation factor 4A2 antibody, IF4A2_HUMAN antibody, N-terminally processed antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

EIF4A2

NCBI Gene ID

1917

UniProt

Q14240

Host

Rabbit

Reactivity

Human and Mouse

Immunogen

Synthetic peptide from the N-terminal region of the human eIF4A2 protein.

Immunogen Type

Human

Target

EIF4A2

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Molecular Weight

46

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~46 kDa eIF4A2 protein.

Formulation

Prepared from pooled rabbit serum by affinity purification via chromatography on peptide immunogen column.

Quality Control

Western blots performed on each lot.

Species

Bovine, Non-Human Primate, Rat

Isotype

IgG

Curated Selection

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