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Anti-Dopamine Transporter, Extracellular Loop 2 Antibody

Our Anti-Dopamine Transporter, Extracellular Loop 2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human and non-human primate Dopamine Transporter, Extracellular Loop 2 and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Specifications

Background

The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999) . Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996) .

Synonyms

DA transporter antibody, DAT 1 antibody, DAT antibody, DAT1 antibody, Dopamine transporter 1 antibody, Dopamine transporter antibody, PKDYS antibody, SC6A3_HUMAN antibody, SLC6A3 antibody, Sodium dependent dopamine transporter antibody, Sodium-dependent dopamine transporter antibody, Solute carrier family 6 (neurotransmitter transporter dopamine) member 3 antibody, Solute carrier family 6 (neurotransmitter transporter) member 3 antibody, Solute carrier family 6 member 3 antibody, Variable number tandem repeat (VNTR) antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

SLC6A3

NCBI Gene ID

6531

UniProt

Q01959

Host

Rabbit

Reactivity

Human and Non-Human Primate

Immunogen

Synthetic peptide corresponding to amino acid residues from the extracellular loop 2 region of human dopamine transporter, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

Dopamine Transporter, Extracellular Loop 2

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Concentration

0.3 mg/mL

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Molecular Weight

80

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

IHC Dilution

1:1000

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~80 kDa DAT protein. Diffuse band may be observed between ~70-85 kDa due to variable glycosylation.

Formulation

Prepared from pooled rabbit serum by affinity purification using a column matrix to which the peptide immunogen was coupled.

Quality Control

Western blots performed on each lot.

Isotype

IgG

Frequently Asked Questions

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