Anti-DGCR8 (Ser377) Antibody

Our Anti-DGCR8 (Ser377) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse DGCR8 (Ser377) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA) -binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han, J., et al, 2006) . The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller, M., et al, 2010) .

Synonyms

DGCRK6 antibody, C22orf12 antibody, D16H22S788E antibody, D16Wis2 antibody, DGCR 8 antibody, Dgcr8 antibody, DGCR8 microprocessor complex subunit antibody, DGCR8_HUMAN antibody, DGCRK 6 antibody, DiGeorge syndrome critical region 8 antibody, DiGeorge syndrome critical region gene 8 antibody, Gy1 antibody, Microprocessor complex subunit DGCR8 antibody, pasha antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

DGCR8

NCBI Gene ID

1644

UniProt

Q8WYQ5

Modification Site

Ser377

Host

Rabbit

Reactivity

Human and Mouse

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 of human DGCR8, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

DGCR8 (Ser377)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

120

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~120 kDa DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.