Anti-DENND3 (Ser554) Antibody

Our Anti-DENND3 (Ser554) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human DENND3 (Ser554) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (Marat, A.L., et al., 2011) . Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (Xu and McPherson, 2015; Matsui et al., 2011) . ULK-mediated phosphorylation of DENND3 at serines 554 and 572 upregulates its GEF activity toward the small GTPase Rab12 (Xu and McPherson, 2015) .

Synonyms

DENN Domain Containing 3, DENN Domain-Containing Protein 3, DENN/MADD Domain Containing 3, KIAA0870 DENN/MADD domain containing 3, KIAA0870DENN domain-containing protein 3

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

DENND3

NCBI Gene ID

315055

UniProt

A2RUS2

Modification Site

Ser554

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser554 of human DENND3, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

DENND3 (Ser554)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

142

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:500

Specificity

Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Ser554. The immunolabeling is completely eliminated by treatment with λ-phosphatase.