Anti-CtIP (Ser327) Antibody

Our Anti-CtIP (Ser327) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human CtIP (Ser327) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

CtIP, C-terminal binding protein-interacting protein, is a DNA endonuclease activated by double stranded breaks (DSBs) . DSB repairs can be performed by either one of two mechanisms; non-homologous end joining (NHEJ) or homologous recombination (HR) . NHEJ is the predominant DSB repair pathway throughout the entire cell cycle, most importantly in the G1 phase (Rothkamm et al, 2003) ; while HR is important for repairing DSBs in S and G2 phases (Beucher et al, 2009) . CtIP controls DSB resection; an event that only occurs in HR during G2-phase. Phosphorylation of Thr-847 dictates the resection efficiency (Huertas et al, 2008) . Furthermore, it has been found that DSBs undergo resection and repair in G1-phase cells via a process requiring Plk3 phosphorylation of CtIP at Ser-327 and Thr-847 (Barton et al, 2014) . Several additional phosphorylation sites within CtIP have been identified, but their significance in the repair of DNA have yet to be determined.

Synonyms

COM1 antibody, COM1_HUMAN antibody, CtBP interacting protein antibody, CtBP-interacting protein antibody, CtIP antibody, DNA endonuclease RBBP8 antibody, JWDS antibody, RB binding protein 8 endonuclease antibody, RBBP-8 antibody, RBBP8 antibody, Retinoblastoma-binding protein 8 antibody, Retinoblastoma-interacting protein and myosin-like antibody, Rim antibody, SAE2 antibody, SCKL2 antibody, Sporulation in the absence of SPO11 protein 2 homolog antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

RBBP8

NCBI Gene ID

5932

UniProt

Q99708

Modification Site

Ser327

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 of human CtIP, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

CtIP (Ser327)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

100

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~100 kDa CtIP protein phosphorylated at Ser327. Immunolabeling is completely eliminated by treatment with λ-phosphatase.