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Anti-CRISPR Cas9 Antibody

Our Anti-CRISPR Cas9 primary antibody from PhosphoSolutions is mouse monoclonal. It detects CRISPR Cas9 and is Protein G purified. It is great for use in WB, IHC, ICC.

Product Specifications

Background

CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015) .

Synonyms

Cas9 antibody, CRISPR-associated endonuclease Cas9/Csn1 antibody, CRISPR-Cas9/Csn1 antibody, csn1 antibody, SpyCas9 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Host

Mouse

Immunogen

Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.

Immunogen Type

Staphylococcus aureus.

Target

CRISPR Cas9

Clonality

Monoclonal

Clone

6F7

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

Lot Dependent

Format

Protein G Purified

Form

Liquid

Buffer

PBS + 50% glycerol and 5 mm NaN3

Molecular Weight

53

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

IHC Dilution

1:1000-1:5000

WB Dilution

1:1000

Specificity

Specific for the Staphylococcus aureus Cas9 protein.

Formulation

Protein G purified culture supernatant.

ICC Dilution

1:500-1:5000

Quality Control

Western blots performed on each lot.

Isotype

IgG1

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