Anti-COXIV (Ser58) Antibody

Our Anti-COXIV (Ser58) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat COXIV (Ser58) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms; COXIV-1 and COXIV-2 (Huttermann et al., 2001) . COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart (Huttermann et al., 2001) . Phosphorylation of amino acid residue Ser-58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation (AcinPerez et al., 2011) .

Synonyms

AL024441 antibody, COX 4 antibody, COX IV 1 antibody, COX IV antibody, COX IV-1 antibody, Cox4 antibody, COX41_HUMAN antibody, Cox4a antibody, COX4B antibody, COX4I1 antibody, COX4I2 antibody, COX4L2 antibody, Cytochrome c oxidase polypeptide IV antibody, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody, Cytochrome C Oxidase subunit IV antibody, Cytochrome c oxidase subunit IV isoform 1 antibody, Cytochrome c oxidase subunit IV isoform 2 (lung) antibody, Cytochrome c oxydase subunit 4 antibody, MGC105470 antibody, MGC72016 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

COX4i1

NCBI Gene ID

12857

UniProt

P19783

Modification Site

Ser58

Host

Rabbit

Reactivity

Mouse and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of mouse COXIV, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Mouse

Target

COXIV (Ser58)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~17 kDa COXIV-1 protein phosphorylated at Ser58. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.