Anti-Connexin 43 (Ser368) Antibody

Our Anti-Connexin 43 (Ser368) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Connexin 43 (Ser368) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001) . Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser-368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a) . Phosphorylation of Ser-368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b) .

Synonyms

Connexin 43 antibody, Connexin-43 antibody, Cx 43 antibody, Cx43 antibody, CXA1_HUMAN antibody, DFNB38 antibody, Gap junction 43 kDa heart protein antibody, Gap junction alpha-1 protein antibody, Gap junction protein alpha 1 43kDa (connexin 43) antibody, Gap junction protein alpha 1 43kDa antibody, Gap junction protein alpha like antibody, GJA 1 antibody, Gja1 antibody, GJAL antibody, ODD antibody, ODDD antibody, ODOD antibody, SDTY3 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

GJA1

NCBI Gene ID

24392

UniProt

P08050

Modification Site

Ser368

Host

Rabbit

Reactivity

Mouse and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Rat

Target

Connexin 43 (Ser368)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~43 kDa connexin-43 protein phosphorylated at Ser368. Immunolabeling is completely eliminated by treatment with λ-phosphatase.