Anti-CK1Mt (Tyr153) Antibody

Our Anti-CK1Mt (Tyr153) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse CK1Mt (Tyr153) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

Creatine kinase (CK1) plays an important role transferring phosphate groups from phosphocreatine to ADP in the cytosol of tissues having high, fluctuating energy demands like skeletal muscle, heart, and brain. Mitochondrial CK1, CK1Mt, has two isoforms, sarcomeric CK1Mt and ubiquitous CK1Mt. CK1Mt is localized between the MIM (mitochondrial inner membrane) and MOM (mitochondrial outer membrane) and bound to the cardiolipin-rich inner leaflet (Muller et al., 1985) and along the cristae membranes (Wegmann et al., 1991) . CK1Mt exists in two forms; a homo dimer and an octamer consisting of four homodimers. The CK1Mt octamer forms permanent contact sites and maintains a complex structure including porin and ANT (adenine nucleotide translocase) within MIM and MOM (Speer et al, 2005) . There have been several phospho-serine, threonine, and tyrosine sites identified within CK1Mt, the role of each one has yet to be determined.

Synonyms

Acidic type mitochondrial creatine kinase antibody, CKMT antibody, CKMT1 antibody, CKMT1A antibody, CKMT1B antibody, Creatine kinase mitochondrial 1 ubiquitous antibody, Creatine kinase U type mitochondrial antibody, Creatine kinase ubiquitous mitochondrial antibody, Creatine kinase mitochondrial 1 (ubiquitous) antibody, Creatine kinase mitochondrial 1A antibody, Creatine kinase mitochondrial 1B antibody, Mia CK antibody, U MtCK antibody, Ubiquitous mitochondrial creatine kinase antibody, UMTCK antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

CKMT1A

NCBI Gene ID

1159

UniProt

P12532

Modification Site

Tyr153

Host

Rabbit

Reactivity

Human and Mouse

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr153 of human CK1Mt, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

CK1Mt (Tyr153)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~46 kDa CK1Mt protein phosphorylated at Tyr153. Immunolabeling of CK1Mt is greatly decreased by lambda-phosphatase treatment.