Anti-Beclin1 (Ser295) Antibody

Our Anti-Beclin1 (Ser295) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Beclin1 (Ser295) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999) . Akt-mediated phosphorylation of beclin-1 at Ser-295 has been shown to decrease autophagy and promote oncogenesis (Wang et al., 2012) .

Synonyms

PG6 antibody, ATG 6 antibody, ATG6 antibody, ATG6 autophagy related 6 homolog antibody, Bcl-2-interacting protein beclin antibody, Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody, Beclin 1 autophagy related antibody, Beclin-1 antibody, Beclin1 antibody, BECN 1 antibody, Becn1 antibody, BECN1_HUMAN antibody, Coiled coil myosin like BCL2 interacting protein antibody, Coiled-coil myosin-like BCL2-interacting protein antibody, GT197 antibody, Protein GT197 antibody, VPS 30 antibody, VPS30 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

BECN1

NCBI Gene ID

8678

UniProt

Q14457

Modification Site

Ser295

Host

Rabbit

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of human beclin-1, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

Beclin1 (Ser295)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:250

Specificity

Specific for endogenous levels of the ~60 kDa beclin1 protein phosphorylated at Ser295. Immunolabeling of the beclin-1 band is eliminated in the beclin-1 alanine substitution mutant.