Anti-ATF2 (Thr52) Antibody

Our Anti-ATF2 (Thr52) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse ATF2 (Thr52) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008) . Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008) . To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr-52 by PKCε regulates this translocation (Lau et al., 2012) .

Synonyms

Activating transcription factor 2 antibody, Activating transcription factor 2 splice variant ATF2 var2 antibody, ATF 2 antibody, Atf-2 antibody, Atf2 antibody, ATF2 protein antibody, ATF2_HUMAN antibody, cAMP Response Element Binding Protein 2 antibody, cAMP response element binding protein CRE BP1 antibody, cAMP response element-binding protein CRE-BP1 antibody, cAMP responsive element binding protein 2, formerly antibody, cAMP-dependent transcription factor ATF-2 antibody, cAMP-responsive element-binding protein 2 antibody, CRE BP1 antibody, CRE-BP antibody, CREB 2 antibody, CREB-2 antibody, CREB2 antibody, CREBP1 antibody, Cyclic AMP dependent transcription factor ATF 2 antibody, Cyclic AMP-dependent transcription factor ATF-2 antibody, Cyclic AMP-responsive element-binding protein 2 antibody, D130078H02Rik antibody, D18875 antibody, HB 16 antibody, HB16 antibody, Histone acetyltransferase ATF2 antibody, MGC105211 antibody, MGC105222 antibody, MGC111558 antibody, MGC142504 antibody, mXBP antibody, MXBP protein antibody, Tg (Gzma-Klra1) 7Wum antibody, TREB 7 antibody, TREB7 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

ATF2

NCBI Gene ID

1386

UniProt

P15336

Modification Site

Thr52

Host

Rabbit

Reactivity

Human and Mouse

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr52 of human ATF2, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

ATF2 (Thr52)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:250

Specificity

Specific for endogenous levels of the ~72 kDa ATF2 protein phosphorylated at Thr52. Immunolabeling of the ATF2 band is reduced by treatment of the cells with the PKC catalytic inhibitor Gö6850.