Anti-Aquaporin 2 (Ser261) Antibody

Our Anti-Aquaporin 2 (Ser261) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Aquaporin 2 (Ser261) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC, IP.

Product Specifications

Background

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007) . A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002) . Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007)

Synonyms

ADH water channel antibody, AQP 2 antibody, AQP CD antibody, AQP-2 antibody, AQP-CD antibody, AQP2 antibody, AQP2_HUMAN antibody, AQPCD antibody, Aquaporin 2 collecting duct antibody, Aquaporin CD antibody, Aquaporin-2 antibody, Aquaporin-CD antibody, Aquaporin2 antibody, Aquaporine 2 antibody, Collecting duct water channel protein antibody, MGC34501 antibody, Water channel aquaporin 2 antibody, Water channel protein for renal collecting duct antibody, WCH CD antibody, WCH-CD antibody, WCHCD antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

AQP2

NCBI Gene ID

25386

UniProt

P34080

Modification Site

Ser261

Host

Rabbit

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261 of rat aquarporin 2, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Rat

Target

Aquaporin 2 (Ser261)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC, IP

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

29/37

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

IHC Dilution

1:2000

WB Dilution

1:1000

IP Dilution

3 ul

Specificity

Specific for endogenous levels of the ~29 kDa AQP2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.