Anti-AIF1/Iba1 Antibody

Our Anti-AIF1/Iba1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse AIF1/Iba1 and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Product Specifications

Background

AIF1, allograft inflammatory factor-1, (also known as Iba1, Ionized calcium binding adaptor molecule 1) is a 17 kDa protein that is a pro-inflammatory molecule that mediates calcium signals (Ito, D. et al 1998) . During inflammation, increased expression of AIF1 occurs in microglia, macrophages, T-cells, synoviocytes, and adipocytes (Watano, K., et al, 2001) . Expression of AIF-1 has been observed in healthy brains and varies throughout the day, gaining the highest expression in the prefrontal and parietal cortices when sleeping (Romcy-Pereira, R.N, et al, 2009) . Increased expression of AIF1 has been observed in several diseases including breast cancer (Liu, S., et al 2008), atherosclerosis (Utans, U., et al, 1995), and rheumatoid arthritis (Kimura, M., et al, 2007) .

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

AIF1

NCBI Gene ID

199

UniProt

P55008

Host

Rabbit

Reactivity

Human and Mouse

Immunogen

Full-length human AIF1/Iba1 protein expressed in and purified from E. coli.

Immunogen Type

Human

Target

AIF1/Iba1

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.