Anti-5-Lipoxygenase (Ser523) Antibody

Our Anti-5-Lipoxygenase (Ser523) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat 5-Lipoxygenase (Ser523) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001) . Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004) . Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004) . Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.

Synonyms

5 Lipoxygenase antibody, 5 LO antibody, 5 LOX antibody, 5-lipoxygenase antibody, 5-LO antibody, 5-LOX antibody, 5LOX antibody, 5LPG antibody, ALOX 5 antibody, Alox5 antibody, Arachidonate 5 lipoxygenase antibody, Arachidonate 5-lipoxygenase antibody, arachidonic 5-lipoxygenase alpha-10 isoform antibody, arachidonic 5-lipoxygenase delta-10-13 isoform antibody, arachidonic 5-lipoxygenase delta-13 isoform antibody, arachidonic 5-lipoxygenase delta-p10 isoform antibody, Arachidonic acid 5 lipoxygenase antibody, Leukotriene A4 synthase antibody, LOG 5 antibody, LOG5 antibody, LOX5_HUMAN antibody, MGC163204 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

ALOX5

NCBI Gene ID

240

UniProt

P09917

Modification Site

Ser523

Host

Rabbit

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser523 of human 5-lipoxygenase (5-LO), conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

5-Lipoxygenase (Ser523)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~80 kD 5-LO phosphorylated at Ser523 in Western blots. Immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-phosphatase, 1200 units for 30 min) .