10X Lysis Buffer
Our 1% SDS lysis buffer from PhosphoSolutions is made in-house. It fully solubilizes membrane and other hard-to-solubilize proteins for WB. Suitable for both organs and tissue culture cells.
Product Specifications
Background
Nonionic detergents such as NP-40 or triton fail to solubilize many cellular proteins involved in cell signaling. This problem is particularly acute in brain where synaptic junctions are known to be insoluble in nonionic detergents. 1% SDS completely solubilizes membrane and other hard to solubilize proteins including synaptic junction proteins. Suitable for both organs and tissue culture cells.
Other Statements
This is a research laboratory reagent and is not for use as diagnostic or therapeutic reagents in humans.
Concentration
10% (w/v) SDS, 100 mM TRIS, 10 mM EDTA, pH 8.0.
Format
SDS Lysis Buffer
Form
Liquid
Buffer
10% (w/v) SDS, 100 mM TRIS, 10 mM EDTA, pH 8.0. Dilute to 1X with dH2O before use.
Shipping Conditions
Ambient
Storage Conditions
Storage at room temperature is recommended for both 10X and 1X solutions to prevent SDS from precipitating out of solution.
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