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Anti-CD133 (Prominin1) Antibody

Our CD133 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human CD133 and is antigen affinity purified. It is great for use in WB, IHC.

Product Specifications

Background

Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Synonyms

PROM1, Antigen AC133, Prominin-like protein 1, PROML1, MSTP061

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

PROM1

UniProt

O43490

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acids from an internal sequence of the human Prominin-1 (CD133) protein.

Immunogen Type

Human

Target

CD133

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB, IHC

Format

Antigen Affinity Purified

Form

Liquid

Buffer

PBS + 0.02% NaN3

Shipping Conditions

Blue Ice

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

IHC Dilution

1:100-1:250

WB Dilution

1:500-1:1000

Specificity

Reacts with an internal sequence of the human Prominin-1 protein. Sequence is 87% identical in mouse and rat.

Formulation

Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Quality Control

Western blots performed on each lot.

Isotype

IgG

Frequently Asked Questions

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