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Anti-Brachyury Antibody

Our Brachyury rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Brachyury and is antigen affinity purified. It is great for use in WB.

Product Specifications

Background

The Brachyury protein is required for the proper formation and differentiation of posterior mesoderm and for axial development in all vertebrates. The Brachyury gene encodes a transcription factor that binds to a specific DNA element via its N-terminal region. A protein motif within the DNA-binding domain, the so-called T box, is highly conserved among T homologs from different species and also defines a broader family of T-box genes Brachyury functions during the earlier stages of gastrulation, which suggests that at least one of its early roles is to regulate the morphogenetic behaviour of nascent mesoderm by altering cell autonomous properties.

Synonyms

TBXT, T-box transcription factor T, Protein T

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

TBXT

UniProt

O15178

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acids from an internal sequence of the human Brachyury protein

Immunogen Type

Human

Target

Brachyury

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB

Format

Antigen Affinity Purified

Form

Liquid

Buffer

PBS + 0.02% NaN3

Shipping Conditions

Blue Ice

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

WB Dilution

1:500-1:1000

Specificity

Reacts with an internal sequence of the human Brachyury protein. Sequence is 80% identical in mouse and rat.

Formulation

Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Quality Control

Western blots performed on each lot.

Isotype

IgG

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