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Anti-Nop30 Antibody

Our Nop30 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Nop30 and is antigen affinity purified. It is great for use in WB.

Product Specifications

Background

Nop30 (nucleolar protein of 30 kDa) is predominantly expressed in muscle and is present in the nucleoplasm and concentrated in nucleoli. The predominant nucleolar localization of Nop30 points to a potential role in RNA processing in muscle nucleoli. A CARD domain containing protein, designated ARC for apoptosis repressor with CARD is related to Nop30 with a different carboxyl terminus.

Synonyms

NOL3, Nucleolar protein 3, Muscle-enriched cytoplasmic protein, Nucleolar protein of 30 kDa, ARC

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

NOL3

UniProt

O60936

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acids from the C-terminal region of the human Nop30 protein

Immunogen Type

Human

Target

Nop30

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB

Format

Antigen Affinity Purified

Form

Liquid

Buffer

PBS + 0.02% NaN3

Molecular Weight

25

Shipping Conditions

Blue Ice

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

WB Dilution

1:500-1:1000

Specificity

Reacts with the C-terminal region of the 25 kDa human Nop30 protein. Since ARC and Nop30 have different C-termini, this antibody is specific for Nop30.

Formulation

Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Quality Control

Western blots performed on each lot.

Isotype

IgG

Curated Selection

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