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Anti-Tet Repressor Antibody

Our Tet Repressor rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects e. coli Tet Repressor and is antigen affinity purified. It is great for use in WB.

Product Specifications

Background

The Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator

Synonyms

Tetracycline repressor protein class D

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

TetR

UniProt

P0ACT4

Host

Rabbit

Reactivity

E. coli

Immunogen

Synthetic peptide corresponding to amino acids from the N-terminal region of E. Coli Tet repressor protein

Immunogen Type

E. Coli

Target

Tet Repressor

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB

Format

Antigen Affinity Purified

Form

Liquid

Buffer

PBS + 0.02% NaN3

Molecular Weight

22

Shipping Conditions

Blue Ice

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

WB Dilution

1:500-1:1000

Specificity

Reacts with the N-terminal region of the 22 kDa Tet repressor protein

Formulation

Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Quality Control

Western blots performed on each lot.

Isotype

IgG

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