Anti-SAP30L Antibody

A novel TGF- beta up-regulated mRNA species, the Sin3-associated protein 30-like, SAP30L has been identified. The predicted nuclear localization signal of SAP30L is sufficient for nuclear transport of the protein although mutating it does not completely remove SAP30L from the nuclei. By reason of its nuclear localization and close homology to SAP30 it is thought that SAP30L might have a role in recruiting the Sin3-histone deacetylase complex to specific co-repressor complexes in response to TGF-beta, leading to the silencing of proliferation-driving genes in the differentiating intestinal epithelial cells.

Histone deacetylase complex subunit SAP30L, HCV non-structural protein 4A-transactivated protein 2, Sin3 corepressor complex subunit SAP30L, Sin3-associated protein p30-like

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Reacts with the C-terminal region of human SAP30L. Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein as well as endogenous hSAP30L in human cell lines (e.g., HEK293 and MCF-7 cells) . Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein in non-human cell lines (e.g., NIH3T3 cells), but does not detect endogenous non-human SAP30L. The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kDa and the endogenous hSAP30L band runs with an apparent molecular weight of 21-22 kDa. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be to cleavage product, degradation and/or product or post-translational modification.