Anti-GAD65/67 (L127/12) Recombinant Chicken Chimeric mAb

Our chicken Anti-GAD65/67 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L127/12. It detects human, mouse, and rat GAD65/67, and is purified by affinity chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

There are two forms of the enzyme glutamic-acid decarboxylase (GAD), a 65-kDa form (GAD65) and a 67-kDa form (GAD67) which are the result of the expression of different genes. Further splicing of GAD 67 produces one or two smaller variants, including GAD25 and GAD44. GAD67 and GAD65 are expressed in adult brain and the pancreas. The truncated forms GAD25 and GAD44 are expressed during embryonic development. Glutamic acid decarboxylase enzyme functions to catalyze the production of gamma-aminobutyric acid from L-glutamic acid.

Synonyms

GAD67, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD1, GAD and DCE1

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

GAD1, GAD, GAD67

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1-594 (full-length) of human GAD67 (accession number Q99259) produced recombinantly in E. Coli

Immunogen Type

Human

Target

GAD65/67

Clonality

Recombinant

Clone

L127/12

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA

Field of Research

Cell Markers Neuronal Markers

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L127/12 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L127/12 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

70 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

IHC Dilution

1:100

WB Dilution

1:1000

Specificity

Cross-reacts with GAD25, GAD44 and GAD65/GAD2

ICC Dilution

1:100

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.