Anti-c-FOS (N486/32) Recombinant Chicken Chimeric mAb (BSA and Azide free)

Our Anti-c-FOS chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone N486/32. It detects human, mouse, and rat c-FOS, and is purified by affinity chromatography. It is great for use in WB, IHC, ICC, ELISA.

Product Specifications

Background

C-fos belongs the Fos family. Other members include FosB, Fra-1, Fra-2. c-fos binds to JUN/AP1 transcription factor. C-Fos is expressed in placental tissue, fetal liver, developing bone, developing central nervous system, and adult bone marrow. It is expressed in the nucleus and in the endoplasmic reticulum. c-Fos has a role in signal transduction, cell proliferation, differentiation, and apoptosis. C-Fos is overexpressed in many cancers.

Short Description

Synonyms

Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

FOS, G0S7

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1-377 (full-length) of human FOS (accession number P01100) produced recombinantly in E. Coli

Immunogen Type

Human

Target

C-FOS

Clonality

Recombinant

Clone

N486/32

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA

Field of Research

Cell Markers Neuronal Markers Activated Neurons Oncoproteins Transcription Regulation

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N486/32 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N486/32 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

PBS

Molecular Weight

70 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when tested by Western Blot.