Anti-Bassoon (L124/59) Recombinant Chicken Chimeric mAb

Our chicken Anti-Bassoon recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L124/59. It detects mouse and rat Bassoon, and is purified by affinity chromatography. It is great for use in AT, IHC, ICC, WB.

Product Specifications

Background

Bassoon is a large (420-kDa) scaffolding protein specifically localized at the active zone of presynaptic nerve terminals. Bassoon is involved in the development of presynaptic terminals and in the regulation of neurotransmitter release at both excitatory and inhibitory brain synapses (Richter K. et al., 1999) .

Synonyms

Protein bassoon

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

Bsn

Host

Chicken

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (accession number O88778) produced recombinantly in E. Coli

Immunogen Type

Rat

Target

Bassoon

Clonality

Recombinant

Clone

L124/59

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA, AT

Field of Research

Cell Markers Neuronal Markers Synapse Markers Pre-synaptic Markers

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L124/59 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L124/59 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

400 kDa for full-length protein, many smaller bands possible depending on sample used

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.