Anti-Calretinin (L122/6) Recombinant Chicken Chimeric mAb

Our chicken Anti-Calretinin recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L122/6. It detects human, mouse, and rat Calretinin, and is purified by affinity chromatography. It is great for use in ICC, IHC, WB.

Product Specifications

Background

Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996) . Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000) .

Short Description

Synonyms

CALB2 (Calretinin) (29 kDa calbindin)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

CALB2 CAB29

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1-178 (N-terminus) of human CALB2 (accession number) produced recombinantly in E. Coli

Immunogen Type

Human

Target

Calretinin

Clonality

Recombinant

Clone

L122/6

Conjugation

Unconjugated

Applications

ELISA, ICC, IHC, WB

Field of Research

Cell Markers Neuronal Markers Immature NeuronsGABAergic Neuronal Markers Cell Signaling

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of our NeuroMab clone L122/6 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L122/6 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

25-35 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.