Anti-Parvalbumin (L114/81) Recombinant Chicken Chimeric mAb

Our chicken Anti-Parvalbumin recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L114/81. It detects mouse and rat Parvalbumin, and is purified by affinity chromatography. It is great for use in AT, ICC, IHC, WB.

Product Specifications

Background

Parvalbumin (PA) is a classical small, mostly cytosolic Ca2+-binding protein of the EF-hand superfamily expressed in vertebrates in a tissue- and cell-specific manner, serving as a magnesium/ calcium buffer (Permyakov EA et al., 2017) . Parvalbumin (PV) -expressing interneurons are a class of inhibitory neurons that fire rapid barrages of action potentials, and are accordingly named “fast-spiking” interneurons (Trachtenberg J. 2015) .

Short Description

Synonyms

Parvalbumin alpha

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

Pvalb Pva

Host

Chicken

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 1-110 (full-length) of rat Parvalbumin (accession number P02625) produced recombinantly in E. Coli

Immunogen Type

Rat

Target

Parvalbumin

Clonality

Recombinant

Clone

L114/81

Conjugation

Unconjugated

Applications

AT, ELISA, ICC, IHC, WB

Field of Research

Cell Markers Neuronal Markers

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of our NeuroMab clone L114/81 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L114/81 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

12 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.