Anti-VGAT (L118/80) Recombinant Chicken Chimeric mAb

Our chicken Anti-VGAT recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L118/80. It detects mouse and rat VGAT, and is purified by affinity chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997) . The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998) . Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005) .

Synonyms

Vesicular inhibitory amino acid transporter (GABA and glycine transporter) (Solute carrier family 32 member 1) (Vesicular GABA transporter) (mVGAT) (mVIAAT)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

Slc32a1 Vgat Viaat

Host

Chicken

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 1-133 (cytoplasmic N-terminus) of mouse VGAT (accession number O35633) produced recombinantly in E. Coli

Immunogen Type

Mouse

Target

VGAT

Clonality

Recombinant

Clone

L118/80

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA

Field of Research

Cell Markers Neuronal Markers Synapse Markers Pre-synaptic Markers Transporter Proteins

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L118/80 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L118/80 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

50 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

IHC Dilution

1:100

WB Dilution

1:500-1:1000

Specificity

No cross-reactivity reported

ICC Dilution

1:100

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.